NABL lab test report for Copper & Argireline® Peptide B'tox Eye Cream

Tested at an NABL-Accredited Laboratory

Copper & Argireline® Peptide B'tox Eye Cream: NABL Lab Test Report

NABL lab test report for Copper & Argireline® Peptide B'tox Eye Cream: heavy metals not detected, pathogens absent, pH 6.42, thermal stability passed. Batch AN6F022.

Testing LaboratorySigma Test & Research Centre
NABL AccreditationTC-15193
Report No.13051827/Z
Batch TestedAN6F022

Safety Summary: Copper & Argireline® Peptide B'tox Eye Cream

Is Copper & Argireline® Peptide B'tox Eye Cream safe? Yes. It was independently tested at Sigma Test & Research Centre, a laboratory accredited by NABL (Accreditation No. TC-15193). The tested sample, from batch AN6F022, was analysed against the limits in the referenced standards.

Free from lead, cadmium, arsenic, mercury and antimony. The report found no detectable lead, cadmium, arsenic or antimony - each below the laboratory's detection limit of 0.25 mg/kg - and no detectable mercury below its detection limit of 0.1 mg/kg.

Free from harmful bacteria and fungi. Three organisms were tested: Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. All were reported Absent. Total microbial count was Less than 10 cfu/gm (limit NMT 100 cfu/gm); Total yeast & mould count was Less than 10 cfu/gm (limit NMT 100 cfu/gm).

Stable under heat. The sample passed the thermal stability test at 45 ± 1°C for 48 hours, and recorded a pH of 6.42 - within the mildly acidic to neutral range suited to the delicate under-eye area.

Is it chemical free? No cosmetic is chemical-free - water itself is a chemical, and this formula is 84.66% water by weight. What this report establishes is that the tested sample is free from any detectable lead, cadmium, arsenic, mercury or antimony and from the listed pathogens.

What these results cover. The laboratory findings apply to the tested sample from batch AN6F022 and the parameters listed on this page. Skin tolerance is assessed separately, in the dermatological patch test certificate. NABL accredits laboratories, not products.

Key Findings at a Glance

🧪
Heavy Metals: Not Detected
Lead, Cadmium, Arsenic, Antimony below 0.25 mg/kg; Mercury below 0.1 mg/kg
🦠
Pathogens: Absent
P. aeruginosa, S. aureus and C. albicans - all reported Absent
📊
Microbial Count: Within Limits
Total count Less than 10 against a limit of NMT 100
🌡️
Thermal Stability: Passed
45 ± 1°C for 48 hours; pH 6.42

Results of Analysis

Result is the measured outcome for this batch. Requirement / Limit is the specification where the standard defines one; a dash means it defines none. Test Method is the standard the laboratory followed. Detection limits (DL) are shown where the laboratory stated one.

Parameter Result Requirement / Limit Test Method
Physical & Organoleptic
Appearance Light green coloured viscous liquid - STRC/STP/F256
Colour Light green - STRC/STP/F256
Odour Characteristic - STRC/STP/P044
Chemical Parameters
pH at 27°C 6.42 - IS:6608:2004
Total residue, % w/w 14.98 - IS:6608:2004, Annex D
Total fatty matter, % w/w 6.84 - IS:6608:2004, Annex C
Thermal Stability (45 ± 1°C for 48 hours) Passes the test - IS:6608:2004, Annex A
Water Content, % w/w 84.66 - IS:2362:1993
Specific Gravity 1.026 - Indian Pharmacopoeia 2022
Heavy Metals
Lead (as Pb), mg/kg Not Detected (DL 0.25) - STRC/STP/P018
Cadmium (as Cd), mg/kg Not Detected (DL 0.25) - STRC/STP/P018
Arsenic (as As), mg/kg Not Detected (DL 0.25) - STRC/STP/P018
Mercury (as Hg), mg/kg Not Detected (DL 0.1) - STRC/STP/P018
Antimony (as Sb), mg/kg Not Detected (DL 0.25) - STRC/STP/P018
Microbial Counts
Total microbial count, cfu/gm Less than 10 NMT 100 IS:14648:2011
Total yeast & mould count, cfu/gm Less than 10 NMT 100 IS:14648:2011
Pathogens
Pseudomonas aeruginosa, per gm Absent Shall be absent IS:14648:2011
Staphylococcus aureus, per gm Absent Shall be absent IS:14648:2011
Candida albicans, per gm Absent Shall be absent IS:14648:2011

Understanding These Tests for Copper & Argireline® Peptide B'tox Eye Cream

Physical & Organoleptic

On the tested sample, Copper & Argireline® Peptide B'tox Eye Cream was recorded as a light green coloured viscous liquid, light green in colour and with a characteristic odour. The light green tint is consistent with a copper peptide formulation.

These checks record what the laboratory observed in the sample as received.

Chemical Parameters

Copper & Argireline® Peptide B'tox Eye Cream recorded pH 6.42 at 27°C, water content 84.66% w/w, total residue 14.98% w/w, total fatty matter 6.84% w/w and specific gravity 1.026. It passed the thermal stability test at 45 ± 1°C for 48 hours.

The thermal stability test exposes the product to sustained heat and checks for separation or phase change - relevant for a water-based gel-cream in Indian transit and storage conditions. The standard sets no numeric specification for the other chemical parameters; they are reported as measured.

Heavy Metals

In this report, Copper & Argireline® Peptide B'tox Eye Cream showed no detectable lead, cadmium, arsenic or antimony (each below 0.25 mg/kg) and no detectable mercury (below 0.1 mg/kg).

Heavy metals can enter a product through raw materials or manufacturing. Note that the copper in this formulation is a peptide complex, not a heavy-metal contaminant, and copper is not among the metals screened here.

Microbial Counts

Copper & Argireline® Peptide B'tox Eye Cream recorded total microbial count Less than 10 cfu/gm against a limit of NMT 100 cfu/gm and total yeast & mould count Less than 10 cfu/gm against a limit of NMT 100 cfu/gm.

The tighter NMT 100 limit for total count reflects the stricter microbiological requirement for products used around the eyes.

Pathogens

In the tested sample of Copper & Argireline® Peptide B'tox Eye Cream, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans were reported Absent - not detected in the tested quantity.

Pathogen tests cover only the organisms listed, not all microorganisms. Escherichia coli was not among the organisms tested in this report.

Test Overview

Report Title Chemical and Microbiological Analysis - Copper & Argireline® Peptide B'tox Eye Cream
Parameters Measured physical and organoleptic properties, chemical parameters including thermal stability, heavy metals, microbial counts and pathogens
Test Type Laboratory chemical and microbiological analysis of a product sample
Test Methods IS:6608:2004; IS:2362:1993; IS:14648:2011; Indian Pharmacopoeia 2022; STRC/STP protocols
Sample Tested AN6F022 (15 g x 12)

About Copper & Argireline® Peptide B'tox Eye Cream

Clinic-Inspired Multi-Peptide Formula | Tightens & Smoothens Under-Eyes in Minutes* | Reduces Wrinkles, Puffiness & Dark Circles | Boosts Collagen & Skin Elasticity. A lightweight gel-cream built around Argireline® and a copper peptide, with caffeine and hesperidin for puffiness. Suitable for all skin types, including sensitive skin. *Brand claim from the product page; not part of the laboratory report.

Claims and Their Evidence

Lab-sourced rows are measured results. Product-page rows are the brand's own claims, shown with the brand's qualifier. The laboratory report is not evidence for cosmetic-benefit claims.

Statement Evidence / basis Source
No detectable heavy metals Lead, Cadmium, Arsenic and Antimony reported Not Detected at a detection limit of 0.25 mg/kg; Mercury Not Detected at 0.1 mg/kg. Lab report 13051827/Z
Within microbiological limits Counts within the standard limits; specified pathogens reported Absent. Lab report 13051827/Z
Thermally stable Passed the thermal stability test at 45 ± 1°C for 48 hours (IS:6608:2004, Annex A). Lab report 13051827/Z
Suitable for all skin types, including sensitive Brand claim on the product page; skin tolerance is assessed in the separate patch test certificate. Product page
Tightens & smoothens under-eyes in minutes* Brand claim on the product page, with the brand's own qualifier. Not tested in this report. Product page

Report Details

Testing Laboratory Sigma Test & Research Centre (NABL-accredited, Accreditation No. TC-15193)
Report No. 13051827/Z
Job Code STRC/13051827
Report Issue Date 22/07/2026
Date of Receipt 10/07/2026
Testing Period 10/07/2026 to 22/07/2026
Batch Tested AN6F022 (Mfg. 06/2026; use before 24 months)
Sample Quantity 15 g x 12
Sample Category Cosmetics (Eye Cream)
Customer Honasa Consumer Limited, Gurugram
Standards Referenced IS:6608:2004; IS:2362:1993; IS:14648:2011; Indian Pharmacopoeia 2022
Authorised Signatory Shruti Vij, Technical Manager, Sigma Test & Research Centre

What This Report Shows

What the results demonstrate

  • Physical characteristics of the tested sample were recorded as observed by the laboratory.
  • The listed heavy metals were below the laboratory's detection limit in the tested sample.
  • The tested sample passed the thermal stability test of the referenced standard.
  • The tested sample met the microbiological count limits of the referenced standard.
  • The specified pathogenic microorganisms were reported Absent in the tested sample.
  • Testing was performed by an NABL-accredited laboratory.

What the results do not demonstrate

  • They do not certify the product; NABL accredits laboratories, not products.
  • They do not establish overall safety or suitability for every individual, and skin tolerance is covered by the separate patch test certificate.
  • They do not prove any cosmetic-benefit or performance claim; those are made on the product page.
  • They do not cover parameters or organisms outside those listed - Escherichia coli and copper content were not tested.
  • They apply to the tested sample from the stated batch, not necessarily to other batches.

Formulation at a Glance

From the actives named on the product page. Not part of the laboratory report.

Key Actives (named on the product page)

Component Role in the formulation
Argireline® (Acetyl Hexapeptide-8) Signalling peptide; the brand's 'B'tox-like' active for expression lines and crow's feet.
Copper Peptide Copper tripeptide complex associated with collagen support; gives the light green tint recorded by the laboratory.
Caffeine Boosts micro-circulation; used against puffiness.
Hesperidin Citrus flavonoid used to support capillary tone and reduce the look of puffiness and dark circles.

Published Research on Key Actives

Published literature on each ingredient, not studies on this product.

Ingredient Published finding Relevance to the formulation
Acetyl Hexapeptide-8 (Argireline®) Topical acetyl hexapeptide-8 reduced wrinkle depth in a 30-day study on periorbital skin (Blanes-Mira C, et al. Int J Cosmet Sci. 2002;24(5):303-310). Supports Argireline® as an expression-line active.
Copper tripeptide (GHK-Cu) GHK-Cu stimulates collagen and glycosaminoglycan synthesis and supports skin remodelling in reviewed studies (Pickart L, Margolina A. Int J Mol Sci. 2018;19(7):1987). Supports the copper peptide as a collagen-support active.
Caffeine Topical caffeine has documented vasoconstrictive and microcirculation effects relevant to puffiness (Herman A, Herman AP. Skin Pharmacol Physiol. 2013;26(1):8-14). Supports caffeine for under-eye puffiness.
Hesperidin Hesperidin shows antioxidant activity and improves skin barrier function in animal and in vitro models (Man MQ, et al. Evid Based Complement Alternat Med. 2019;2019:2676307). Supports hesperidin as a supporting active.

Dermatological Patch Test Certificate

This NABL lab test report covers the chemical and microbiological analysis of Copper & Argireline® Peptide B'tox Eye Cream. Skin tolerance is a separate question, assessed through dermatological patch testing. The dermatological patch test certificate for this product is published on its own page, so you can review the laboratory analysis here and the patch-test findings there as one trust cluster.

View the Original Lab Report

The complete original report for Copper & Argireline® Peptide B'tox Eye Cream is available as issued by the laboratory. It carries the laboratory letterhead, the report number, and the authorised signatory's details.

View Report PDF

The Tested Product

Copper & Argireline® Peptide B'tox Eye Cream

Copper & Argireline® Peptide B'tox Eye Cream

The results on this page come from laboratory testing of a sample from batch AN6F022. See the complete ingredient list, current offers and reviews on the product page.

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Frequently Asked Questions

Q: Is Copper & Argireline® Peptide B'tox Eye Cream tested for heavy metals?

A: Yes. In this report, Lead, Cadmium, Arsenic and Antimony were reported below the laboratory's stated detection limit of 0.25 mg/kg, and Mercury below 0.1 mg/kg, at Sigma Test & Research Centre, an NABL-accredited laboratory (Accreditation No. TC-15193).

Q: Is it safe to use near the eyes?

A: For the tested batch, microbial counts were within the NMT 100 cfu/gm limit applied to eye-area products and the specified pathogens were Absent. Skin tolerance around the eye is assessed separately in the dermatological patch test certificate. As with any eye cream, keep it out of the eye itself.

Q: Does the copper in the name mean it contains heavy metals?

A: No. The copper is a copper peptide complex added deliberately as an active, at cosmetic levels, and is responsible for the light green tint the laboratory recorded. The heavy-metal screen on this page covers lead, cadmium, arsenic, mercury and antimony - contaminants, not intended actives - and none were detected.

Q: Is it stable in Indian heat?

A: The tested sample passed the thermal stability test at 45 ± 1°C for 48 hours under IS:6608:2004. This checks for separation or phase change under sustained heat.

Q: Does this report prove the eye cream works?

A: No. The report covers chemical and microbiological parameters on a sample from the stated batch. Performance claims such as tightening or reducing dark circles are made by the brand on the product page and are not part of this laboratory report.

Q: What do "Not Detected" and "Absent" mean in this report?

A: "Not Detected" means a substance was below the laboratory's stated detection limit using the specified method. "Absent" means a specified microorganism was not detected in the tested quantity using the referenced method.

Q: Does NABL certify this product?

A: No. NABL accredits laboratories, not products. This page reports the results of testing performed at Sigma Test & Research Centre, an NABL-accredited laboratory (Accreditation No. TC-15193) on a sample of this product. It does not represent a product certification by NABL.

Q: Which batch does this report cover?

A: The report covers batch AN6F022 (manufactured 06/2026, use before 24 months). The laboratory report states the tested sample as 15 g x 12. Results refer only to the tested sample and the parameters listed.

References

  1. IS:6608:2004. Methods of test for cosmetics - skin creams. Bureau of Indian Standards.
  2. IS:2362:1993. Determination of water by Karl Fischer method. Bureau of Indian Standards.
  3. IS:14648:2011. Cosmetics - Microbiology - Enumeration and detection of microorganisms. Bureau of Indian Standards.
  4. Indian Pharmacopoeia 2022. Indian Pharmacopoeia Commission.
  5. Blanes-Mira C, et al. A synthetic hexapeptide (Argireline) with antiwrinkle activity. Int J Cosmet Sci. 2002;24(5):303-310.
  6. Pickart L, Margolina A. Regenerative and protective actions of the GHK-Cu peptide. Int J Mol Sci. 2018;19(7):1987.
  7. Sigma Test & Research Centre. Test report 13051827/Z, Job Code STRC/13051827. 2026.

Report Verification

Testing Laboratory

Sigma Test & Research Centre

NABL Accreditation No.

TC-15193

Report No.

13051827/Z

Job Code

STRC/13051827

Batch Tested

AN6F022 (Mfg. 06/2026)

Report Date

22/07/2026

Results refer only to the tested sample from the stated batch and the parameters listed on this page.

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Disclaimer: This page reproduces the results of laboratory analysis performed at Sigma Test & Research Centre on a sample from batch AN6F022 of Copper & Argireline® Peptide B'tox Eye Cream. Test results refer only to the tested sample and the listed parameters. Formulations may be updated over time; refer to the product label for current information. This page is for informational purposes and does not constitute medical or regulatory advice, and does not represent a product certification by NABL.